It allows for the direct visual comparison between the sample and a known standard, helping to identify. The order of increasing rf values in tlc is benzil, methanol anthracene and tryphenyl Response details:1) factor that affects the rf value of tlc is the polarity of the solvent. The polarity of a tlc plate is important in chromatography because it affects how different compounds move and separate on the plate. That depends on many factors including the elution solvent used, the type of tlc plate used, etc. When using.5% of acetic acid in ethyl acetate as your elution solvent and on a plastic backed.
It doesn't depends upon the tlc plates. As it depends upon the solvents in which these compounds migrate. Ninhydrin is used in amino acid tlc because it readily stains amino acids by reacting with the amine groups It is necessary to run tlc ina closed container and have the interior saturated with the solvent vapour to ensure maximum resolution between components and to prevent solvent evapourating off
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