The oxygen dissociation curve (odc) describes the relationship of oxygen tension and haemoglobin oxygen saturation. Physoxic protocols allow researchers to simulate the oxygen environment of healthy tissues, enhancing the relevance of studies on normal cell function and development Hypoxic protocols, on the other hand, provide a framework for investigating the stress responses associated with oxygen deprivation. In the ex vivo experiment, a close loop blood circulation system was built, precisely controlling the oxygen saturation and the hemoglobin concentration of a liquid phantom. Assessing intravascular blood oxygen saturation (so2) is crucial for characterizing in vivo microenvironmental changes in preclinical models of injury and disease. Research in this area primarily investigates the degradation of either hemoglobin or genetic material over time
The optical probe uses white light spectroscopy to record the time dynamics of blanching and refilling The magnitude and dynamics of changes in blood content and hemoglobin oxygen saturation yield an estimate of the oxygen consumption rate (ocr) in units of attomoles per cell per second.
OPEN
