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If the key genes involved in the folic acid metabolism pathway are mutated, the activity of folic acid metabolizing enzymes will be reduced, resulting in folic acid metabolism disorder.

This review summarizes and analyzes the current research on folic acid synthesis and the key issues currently facing biosynthesis limitations, and proposes the development direction and feasibility recommendations for the efficient biosynthesis of folic acid based on synthetic biology strategies. A convenient small scale chemical synthesis of double labeled folic acid with 13 c at positions c‐7 and c‐9 is reported [1,3‐ 13 c 2] acetone was converted into folic acid in two steps, with [1, 1, 3]‐trichloroacetone as the labeled intermediate. Folic acid synthesis method abstract the present invention relates to a folic acid synthesis method. Three types of folate and antifolate cellular transporters have been found, differing in tissue localization, substrate affinity, type of transferring, and optimal ph for function Laboratory criteria of folate deficiency are accepted by who.